ABSTRACT
OBJECTIVE To investigate the component characteristics and anti-infla mmatory activities of the low polarity parts from Sabia campanulata ，S. discolor and S. parviflora . METHODS Gas chromatography-mass spectrometry was used to analyze the components of low polarity parts eluted by petroleum ether from petroleum ether extract of dried stems and leaves of S. campanulata，S. discolor and S. parviflora . The chromatographic peaks were retrieved by both NIST 17 and Wiley 275 database， and their structures of each compound were compared and identified manually. The relative percentage content of each component was calculated by peak area normalization method. With indomethacin as positive control and the inhibition rate of nitric oxide （NO）production as the index ，the effects of the low polarity parts of the above three species of Sabia genus on the production of NO in lipopolysaccharide-induced human rheumatoid arthritis fibroblast-like synoviocytes （RA-FLS） and mouse mononuclear macrophage RAW 264.7 were compared ；the half inhibitory concentration （IC50）was calculated. RESULTS Totally 70，40 and 33 chemical components were identified respectively from the low polarity parts of S. campanulata ，S. discolor and S. parviflora ， including long-chain fatty hydrocarbons ，long-chain alcohol/aldehyde/ester ，monoterpenes；but there were only 5 common components in the three species ，such as dodecane and docosane，etc. S. campanulata contained abundant terpenoids （40.89％）； the relative percentage content of triterpenoids was high in S. discolor （30.29％）；the relative percentage content of long-chain fatty hydrocarbons was high in S. parviflora （87.70％）. The values of IC 50 of the low polarity parts of the above three species of Sabia genus to the production of NO in RA-FLS cells were 2.22，1.69 and 4.15 μg/mL，respectively. The values of IC 50 of them to the production of NO in RAW 264.7 cells were 1.23，4.02 and 3.05 μg/mL respectively，and all of them were lower than indomethacin （12.97，10.66 μg/mL）. CONCLUSIONS The components of the low polarity parts fr om the above three species of Sabia genus are mainly long-chain fatty hydrocarbons ， monoterpenes and long-chain alcohol/aldehyde/ester ， and all of them have certain anti-inflammatory activities.
ABSTRACT
Objective:To explore the use of Delphi method and superiority chart to construct flipped classroom teaching index evaluation system.Methods:Delphi method was applied to conduct expert consultation, and the superiority chart was used to calculate the weight of each index of the flipped classroom teaching evaluation system. SPSS 17.0 was used to statistically analyze the importance assignment of indicators.Results:After two rounds of correspondence consultation, the evaluation system of flipped classroom teaching mode was established, with 3 first-level indicators with weights of (0.32, 0.56 and 0.12); 6 second-level indicators with weights of (0.45, 0.55, 0.56, 0.44, 0.55 and 0.45) and 20 third-level indicators.Conclusion:Delphi method can not be substituted for other methods of mathematical statistics. In terms of weight determination, compared with analytic hierarchy process (ahp), the superiority chart does not need to establish judgment matrix, or to conduct consistency test. This method is simple to operate, time-saving and labor-saving, and is worthy of extensive promotion.
ABSTRACT
Objective To investigate the clinicopathological features of undifferentiated pancreatic carcinoma with osteoclast-like giant cells (UOC).Methods Clinical and pathological data of 6 patients with UOC admitted in Navy Medical University affiliated Changhai Hospital from April 2011 to December 2017 were retrospectively analyzed.The expressions of Cam5.2,EMA,CD68,vimentin,lysozyme,E-cad,C-erbB-2,p53,MUC1,MLH1,MSH2,MSH6,Osteoponin and Ki-67 were detected by immunohistochemistry,and Kras gene mutations was measured by fluorescence quantitative PCR method.Results There were two main types of UOC cells.One type was oval or spindle mononuclear tumor cells,and the other was osteoclast-like giant cells scattered among mononuclear cells.Immunohistochemical staining results showed that the epithelial markers like CAM5.2,EMA,E-cad,MLH1,MSH2,MSH6 and Ki-67 of the components of ductal adenocarcinoma in 6 UOC patients were all positively expressed,and the mesenchymal markers like vimentin,CD68,lysozyme and tumor-related markers like Her-2,p53 and MUC1 were all negatively expressed.Vimentin,CD68,lysozyme and Osteoponin were positively expressed in osteoclast-like giant cells,but epithelial markers like Cam5.2,EMA,E-cad,MLH1,MSH2 and MSH6 were negatively expressed.6 UOC patients all had K-ras codon 12 mutation,and the mutant type was GGT ＞ TGT,GGT ＞ GTT,GGT ＞ GCT,and no codon 13 mutation was observed.Conclusions Osteoclast-like giant cells may be reactive multinucleated giant cells rather than neoplastic components.
ABSTRACT
Objective To analyze the application of clinicopathological features and LEF-1 in the diagnosis of solid pseudopapillary neoplasm of the pancreas (SPN).Methods Clinical and pathological data of 227 cases who were pathologically diagnosed as pancreatic SPN at Changhai Hospital from Jan 2000 to Dec 2015 were collected and analyzed.Immunochemical assay was used to detect the expression of LEF-1 in 132 cases of SPN, and the results were compared with β-catenin, which is most commonly used for diagnosing SPN.Results 81.9% of patients with SPN were female (186/227).Mean age at the onset was 34 years.Mean tumor size was 5.4 cm.48.5% tumors were localized in the pancreatic tail, and 33% in the head.46.3% tumors were cystic and solid, 42.3% were solid, and 11.4% were cystic.There were 2 cases of lymph node metastasis (0.9%), 15 cases of vascular tumor thrombus (6.6%), 14 cases of nerve invasion (6.2%), and 13 cases of adjacent organs invasion (5.7%) based on microscopic observations.Immunochemical analysis showed that 130 of 132 cases with SPN expressed LEF-1 with strong nuclear positivity, and the positivity rate was 98.5%.But no obvious expression of LEF-1 can be seen in normal pancreatic tissue and other pancreatic tumors.The specificity was 100%.The positivity of β-catenin expression in SPN was 96.6%(144/149), and β-catenin was positively expressed in only one case of acinar cell carcinoma.Tumors were completely removed by surgery in 165 cases, and the median follow-up was 51 months.By Oct 31, 2016, 162 patients (98.2%) survived, 5 had liver metastasis, and 1 had recurrence.Conclusions SPN is predominantly encountered in young female patients, and the clinical manifestations are not specific.LEF-1 can be used as a specific marker for the diagnosis and differentiation of SPN, which is more accurate than β-catenin.
ABSTRACT
Objective To explore how to use Epidata and SPSS software in process of coding,entry and statistical analysis of multiple choice conveniently and quickly.Methods On the basis of the hospital demands for nursing graduates behavior questionnaire,Epidata and SPSS software was adopted to realize information coding,entry and statistical analysis of multiple choice,directional selection problem,sort multiple-choice questions.Results Through examples show,a combination of the two software carried on reasonable coding,entry and processing in the three different types of multiple-choice questions,involved in the hospital demands for nursing graduates behavior questionnaire,and gave registration interface of Epidata software,multiple choice encoding and SPSS data analysis process.Conclusions The combination of the two kinds of software can greatly improve the speed of data entry,especially when the data sample is large.
ABSTRACT
Objective To argue whether the Chinese version of Emotional Intelligence Scale (EIS) can be used to evaluate emotional intelligence of nursing students through studying its reliability and validity,and provide basis for the study of emotional intelligence of nursing students.Methods Using stratified random sampling method,Chinese version of EIS compiled by Wang Caikang was used to carry out the questionnaire survey among university and college students of different nursing specialty,using Cronbach coefficient alpha and factor analysis method to study the reliability and validity.Results Each dimension and total reliability of the Chinese version of EIS were 0.735,0.815,0.801,0.790,0.821.Through the principal component analysis,four common factors were extracted,the cumulated variance contribution of the four factors was 55.468％.Conclusions Chinese version of EIS has good reliability and validity,which is fully applicable to nursing students' emotional intelligence research.
ABSTRACT
Objective To establish a CLCN3/MMTV-PyMT double transgenic mouse model of spontaneous breast tumor with simultaneously overexpressing ClC-3.Method CLCN3 transgenic mice were crossed with MMTV-PyMT spon-taneous mammary tumor model mice.The genotype was determined by PCR.The expression of ClC-3 in tissues was detec-ted by immunofluorescence and Western blot.Results CLCN3 and MMTV-PyMT transgenic mice were bred and CLCN3/MMTV-PyMT hybrid mouse model was successfully established.The ClC-3 expression in CLCN3/MMTV-PyMT hybrid mice was higher than that in the MMTV-PyMT mice, assessed by immunofluorescence and Western blot analysis.Conclu-sions Transgenic mouse models of spontaneous breast cancer with simultaneously overexpressing ClC-3 are successfully es-tablished.The double transgenic mice provide a good animal model for further research of ClC-3 in tumor growth and metas-tasis.
ABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of Periploca forrestii.</p><p><b>METHOD</b>The constituents were separate using such various column chromatographic techniques as silica gel, RP-18 silica gel, MCI and Sephadex LH-20. Their structures were identified by such methods as spectral analysis.</p><p><b>RESULT</b>Ten compounds were isolated and identified as periforgenin A-3-O-beta-digitoxopyranoside (1), beta-sitosterol (2), periforoside I (3), ursolic acid (4), periplogenin (5), periplocin (6), glycoside E (7), periplocoside M (8) , daucosterol (9), 2alpha, 3alpha, 23-trihydroxy-urs-12-en-28-oic acid (10).</p><p><b>CONCLUSION</b>Compound 1 was a new cardiac glycoside and compound 8 was reported for the first time from this plant.</p>